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Abstract

Local black goat are raised for the purpose of milk production. The goal of the current study was to identify the growth hormone gene's single nucleotide polymorphism (SNPS). Additionally, the effect of the GH gene SNP on the milk qualities of Iraqi black goat was evaluated.  DNA was extracted from blood samples of 28 goat. A segment with a length of 330 bp in the third intron and the fourth exon of the GH gene was analyzed. Bioinformatics software was used to analyze the results. The results showed the presence of three genotypes (TT, TC, CC) at position 263 of the studied plot with a frequency of 0.36, 0.21, and 0.43, respectively. The local breed was characterized by a decrease in observed heterozygosity (21.43%). However, the expected heterozygosity was 50.65%. Low heterozygosity may indicate the presence of inbreeding within the same herd. Haplotypes network revealed a genetic relationship between the local Iraqi goat and the Egyptian goat. As well as the existence of a genetic relationship at a lower level with the Indian goats. From Tajima's D test and Fu's Fs (1.547 and 1.428), the results led to a rise in inbreeding. In milk production, energy, and the amounts of protein. The analysis of the data using bioinformatics tools predicted the effects of identified the genotypes in which the amino acid was not altered, and this is where the mutation was found. There is a relationship between the genetic polymorphism of the growth hormone gene and the production of milk and its components. The CC genotype was superior in milk production, energy, and the amounts of protein and fat over the TC and CC combinations.

Keywords

Domestic goat GH gene Milk Yield Single Nucleotide Polymorphism

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Yousief, M. Y. ., Owaid , J. M. ., & Mohsin , R. H. . (2023). Relationship Between Growth Hormone Gene Polymorphism with Milk Production and Its Components in Black Iraqi Goat. Basrah Journal of Agricultural Sciences, 36(2), 285–299. https://doi.org/10.37077/25200860.2023.36.2.22
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References

  1. Al-Hassnawi, I. A. S., & Ayied, A. Y. (2022a). Detection of genetic diversity of local Iraqi black goats by using Cytochrome b gene. NeuroQuantology|, 20(8), 7626-7634.
  2. https://www.neuroquantology.com/open-access/Detection+of+genetic+diversity+of+local+Iraqi++black+goats+by+using+Cytochrome_4019/
  3. Al-Hassnawi, I. A. S., & Ayied, A. Y. (2022b). Detection of genetic diversity of local Iraqi black goats by using D-loop Region. NeuroQuantology, 20(8), 7642-7651.
  4. https://www.neuroquantology.com/open-access/Detection+of+genetic+diversity+of+local+Iraqi++black+goats+by+using+D-loop+Region_4022/
  5. Al-Hubaety, A. K., & Al-Juwari, M. F. (2021). The effect of milking methods on milk production and its components and the relationship between milk components and biochemical characteristics of blood in Awassi sheep. Journal of Agricultural, Environmental and Veterinary Sciences, 5(2), 15-27.
  6. https://journals.ajsrp.com/index.php/jaevs/article/view/3809
  7. Al-Qasimi, R. H., Hassan, A. F., & Khudair, B. Y. (2019). Effect of IGF-1 and GH genes polymorphism on weights and body measurements of Awassi lambs in different ages. Basrah Journal of Agricultural Sciences, 32(1), 39-46.
  8. https://doi.org/10.37077/25200860.2019.125
  9. Al-Salihi, A., Al-Saadi, B., & Al-Anbari, N. (2017). Genotypes relationship of Growth hormone gene polymorphism with some productive and reproductive trait in Awassi sheep. Journal of Biotechnology Research Center, 11(2). (In Arabic)
  10. https://doi.org/10.24126/jobrc.2017.11.2.516
  11. Al-Shuhaib, M. B. S. (2019). A comprehensive in silico prediction of the most deleterious missense variants in the bovine LEP gene. Journal of Biotechnology, Computational Biology and Bionanotechnology. 100(4C), 429–439.
  12. https://doi.org/10.5114/bta.2019.90244
  13. Al-Shuhaib, M. B. S., Al-Thuwaini, T. M., Fadhil, I. A., & Aljubouri, T. R. S. (2019). GHRL gene-based genotyping of ovine and caprine breeds reveals highly polymorphic intronic sequences in Awassi sheep with several RNA motifs. Journal of Genetic Engineering and Biotechnology, 17(3), 1-8.
  14. https://doi.org/10.1186/s43141-019-0004-5
  15. Aris-Brosou, S., & Excoffier, L. (1996). The impact of population expansion and mutation rate heterogeneity on DNA sequence polymorphism. Molecular biology and evolution, 13(3), 494-504.
  16. https://academic.oup.com/mbe/article/13/3/494/1057318
  17. Bekele, R., Taye, M., Abebe, G., & Meseret, S. (2023). Genomic regions and candidate genes associated with milk production traits in Holstein and its crossbred cattle: A review. International Journal of Genomics, 2023, 1-18.
  18. https://doi.org/10.1155/2023/8497453
  19. Falaki, M., Prandi, A., Corradini, C., Sneyers, M., Gengler, N., Massart, S., Fazzini, U., Burny, A., Portetelle, D., & Renaville, R. (1997). Relationships of growth hormone gene and milk protein polymorphisms to milk production traits in Simmental cattle. Journal of Dairy Research, 64(1), 47-56.
  20. https://doi.org/10.1017/s0022029996001872
  21. Fu, Y.-X. (1997). Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics, 147(2), 915-925.
  22. https://doi.org/10.1093/genetics/147.2.915
  23. Gholamhoseinzadeh Gooki, F., Mohammadabadi, M., & Asadi Fozi, M. (2018). Polymorphism of the growth hormone gene and its effect on production and reproduction traits in goat. Iranian Journal of Applied Animal Science, 8(4), 653-659.
  24. https://ijas.rasht.iau.ir/article_544783.html
  25. Hall, M. B. (2023). Invited review: Corrected milk: Reconsideration of common equations and milk energy estimates. Journal of dairy science.
  26. https://doi.org/10.3168/jds.2022-22219
  27. Hill, W. G. & Mackay, T. F. C (2004). D. S. Falconer and Introduction to Quantitative Genetics, Genetics, 167(4),1529-1536.
  28. http://doi.org/10.1093/genetics/167.4.1529
  29. Jaffar, A. A., Hassan, A. F., & Kassim, W. Y. (2019). Effect of pou1f1 gene haplotypes on eights and milk production of awassi sheep. Basrah Journal of Agricultural Sciences, 32(2), 85-94.
  30. https://doi.org/10.37077/25200860.2019.199
  31. Kimura, M., & Crow, J. F. (1964). The number of alleles that can be maintained in a finite population. Genetics, 49(4), 725.
  32. https://doi.org/10.1093/genetics/49.4.725
  33. Kioka, N., Manabe, E., Abe, M., Hashi, H., Yato, M., Okuno, M., Yamano, Y., Sakai, H., Komano, T., & Utsumi, K. (1989). Cloning and sequencing of goat growth hormone gene. Agricultural and Biological Chemistry, 53(6), 1583-1587.
  34. https://doi.org/10.1080/00021369.1989.10869514
  35. Kojima, M., Hosoda, H., Date, Y., Nakazato, M., Matsuo, H., & Kangawa, K. (1999). Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature, 402, 6762, 656-660.
  36. https://doi.org/10.1038/45230
  37. Kunda, R. M., Volkandari, S. D., Rumanta, M., & Kakisina, P. (2020). Polymorphism of growth hormone (GH) gene in lakor goat from Lakor Island of Southwest Maluku regency. Buletin Peternakan, 44(4), 233-238.
  38. https://doi.org/10.21059/buletinpeternak.v44i4.58934
  39. Lewontin, R. C. (1974). The genetic basis of evolutionary change. Columbia University Press, New York, 346pp.
  40. https://doi.org/10.1086/288688
  41. Lukes, A., Barnes, M., & Pearson, R. (1989). Response to selection for milk yield and metabolic challenges in primiparous dairy cows. Domestic Animal Endocrinology, 6(4), 287-298.
  42. https://doi.org/10.1016/0739-7240(89)90023-4
  43. Malveiro, E., Pereira, M., Marques, P., Santos, I., Belo, C., Renaville, R., & Cravador, A. (2001). Polymorphisms at the five exons of the growth hormone gene in the algarvia goat: possible association with milk traits. Small Ruminant Research, 41(2), 163-170.
  44. https://doi.org/10.1016/S0921-4488(01)00198-5
  45. Marques, P., Pereira, M., Marques, M., Santos, I., Belo, C., Renaville, R., & Cravador, A. (2003). Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat. Small Ruminant Research, 50(1-2), 177-185.
  46. https://doi.org/10.1016/S0921-4488(03)00104-4
  47. Moneva, C. S. O., Vega, R. S., Sangel, P. P., Angeles, A. A., & Mendioro, M. S. (2020). Genetic variability in the growth hormone gene (A781G) and its association with milk yield performance in crossbred anglo-nubian dairy goats. Philippine Journal of Science, 149(3).
  48. https://www.ukdr.uplb.edu.ph/journal-articles/508/
  49. Mousavizadeh, A., Mohammad Abadi, M., Torabi, A., Nassiry, M. R., Ghiasi, H., & AliEsmailizadeh Koshkoieh, A. (2009). Genetic polymorphism at the growth hormone locus in Iranian Talli goats by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Iranian Journal of Biotechnology, 7(1), 51-53.
  50. https://www.ijbiotech.com/article_7064.html
  51. Nguyen, V. D., Nguyen, C. O., Chau, T. M. L., Nguyen, D. Q. D., Han, A. T., & Le, T. T. H.. (2023). Goat production, supply chains, challenges, and opportunities for development in Vietnam: A Review. Animals, 13(15), 2546.
  52. https://doi.org/10.3390/ani13152546
  53. Owaid, J. M., Yousief, M., Abdulrda, A., & Ayied, A. (2023). Study of local black Iraqi goats genotypes for the cytb gene. Archives of Razi Institut, 78(3), 915-921.
  54. https://doi.org/10.22092/ari.2022.359888.2499
  55. Paetkau, D., Calvert, W., Stirling, I., & Strobeck, C. (1995). Microsatellite analysis of population structure in Canadian polar bears. Molecular Ecology, 4(3), 347-354.
  56. https://doi.org/10.1111/j.1365-294X.1995.tb00227.x
  57. Pollott, G., & Gootwine, E. (2004). Reproductive performance and milk production of Assaf sheep in an intensive management system. Journal of dairy science, 87(11), 3690-3703.
  58. https://doi.org/10.3168/jds.S0022-0302(04)73508-0
  59. Ruzina, M., Shtyfurko, T., Mohammadabadi, M., Gendzhieva, O., Tsedev, T., & Sulimova, G. (2010). Polymorphism of the BoLA-DRB3 gene in the Mongolian, Kalmyk, and Yakut cattle breeds. Russian Journal of Genetics, 46, 456-463.
  60. https://doi.org/10.1134/S1022795410040113
  61. Schlee, P., Graml, R., Schallenberger, E., Schams, D., Rottmann, O., Olbrich-Bludau, A., & Pirchner, F. (1994). Growth hormone and insulin-like growth factor I concentrations in bulls of various growth hormone genotypes. Theoretical and Applied Genetics, 88, 497-500.
  62. https://doi.org/10.1007/BF00223667
  63. Singh, P., Tomar, S., Thakur, M., & Kumar, A. (2015). Polymorphism and association of growth hormone gene with growth traits in Sirohi and Barbari breeds of goat. Veterinary World, 8(3), 382–387. Abstract Aim: The aim was to study the polymorphism of exon, 2.
  64. https://doi.org/10.14202%2Fvetworld.2015.382-387
  65. SPSS (2019) IBM Statistics 26 step by step. 16th Edition. https://doi.org/10.4324/9780429056765
  66. Tajima, F. (1989). Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics, 123(3), 585-595.
  67. https://doi.org/10.1093/genetics/123.3.585
  68. Todaro, M., Scatassa, M. L. & Giaccone, P. (2005). Multivariate factor analysis of Girgentana goat milk composition. Italian Journal of Animal Science, 4(4), 403-410.
  69. https://doi.org/10.4081/ijas.2005.403
  70. Valinsky, A., Shani, M., & Gootwine, E. (1990). Restriction fragment length polymorphism in sheep at the growth hormone locus is the result of variation in gene number. Animal Biotechnology, 1(2), 135-144.
  71. https://doi.org/10.1080/10495399009525736
  72. Yamano, Y., Oyabayashi, K., Okuno, M., Yato, M., Kioka, N., Manabe, E., Hashi, H., Sakai, H., Komano, T., & Utsumi, K. (1988). Cloning and sequencing of cDNA that encodes goat growth hormone. FEBS letters, 228(2), 301-304.
  73. https://doi.org/10.1016/0014-5793(88)80020-6
  74. Yardibi, H., Hosturk, G. T., Paya, I., Kaygisiz, F., Ciftioglu, G., Mengi, A., & Oztabak, K. (2009). Associations of growth hormone gene polymorphisms with milk production traits in South Anatolian and East Anatolian Red cattle. Journal of Animal and Veterinary Advances, 8(5), 1040-1044.
  75. https://medwelljournals.com/abstract/?doi=javaa.2009.1040.1044
  76. Yousif, A. N., & Mohammed, A. (2022). Genetic diversity of Iraqi local goat breeds by RAPD DNA markers. Jurnal Kedokteran Hewan, 16(4), 127-131.
  77. https://doi.org/10.21157/j.ked.hewan.v16i4.28579
  78. Yousif, A. N., Mohammed A. K., Mahmmud B. M., & Juma F. T. (2011). Effect of parturition and lactation on some haematological and biochemical characteristics in Mountain Black goat. Journal of Kirkuk University for Agricultural Sciences, 2(1):57-65.
  79. https://kujas.uokirkuk.edu.iq/article_33395.html