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An accurate identification of an insect is essential for the effective pest management program. However, due to the restrictions of phenotypic identification, molecular process (e.g. DNA barcoding) is used to overcome some of these limitations. The present paper was aimed to identify the Jujube fruit fly by morphological characters of the adults and molecular DNA barcoding of the larval stage in southeast Iraq. The adults was morphologically identified as Carpomyia incompleta (Becker, 1903) (Diptera: Tephritidae). Results of the molecular method revealed that the mitochondrial COI – COII marker was successfully identified immature stage of the species. The sequence analysis results identified the specimens (AHL1 and AHL2) as C. incompleta. Sequences of the specimens were recorded in The US National Center for Biotechnology Information (NCBI) under accession numbers (ON045002.1 and ON045003.1, respectively. These results were consistent with the phenotypic identification of the Jujube fruit flies.


Carpomya incompleta Fruit flies Identification Jujube Zizaphus spp.

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How to Cite
Taher , H. M. ., & Alyousuf, A. . (2023). Morphological and Molecular Identification of Jujube Fruit Fly Carpomya incompleta (Becker, 1903) (Diptera: Tephritidae) southern Iraq. Basrah Journal of Agricultural Sciences, 36(1), 39–49.


  1. Abdel-Galil, F. A., Rizk, M. M., Temerak, S. A., & Darwish, D. Y. (2014). Studies on the parasitoid Opius concolor Sźepligeti (Hymenoptera: Braconidae), associated with zizyphus fruit fly, Carpomyia incompleta Becker (Diptera: Tephritidae). Archives of Phytopathology and Plant Protection, 47(6), 665-674
  3. Al-Yousuf, A., & Al-Miahy, M. (2007). Effect of calcium on Jujube Ziziphus sp. fruit growth and their resistance to jujube fruit fly carpomyia incompleta. Journal of Kerbala University, 3(2), 106-113.
  4. Alyousuf, A. A.; Abass, M. H. ; Maziel, M. M. (2004). Seasonal appeararnce and feeding preference of butterfly of Jujubes leaves Tarucus rosaceus (Austat) [ Lycaenidae ; Lepidoptera ] on different cultivars of Jujbe Ziziphus spp. Basrah Journal of Agricultural Sciences, 17(2), 187-197.
  5. Aluja, M., & Norrbom, A. (1999). Fruit flies (Tephritidae): phylogeny and evolution of behavior: Crc Press. 984 pp.
  7. Ball, S. L., & Armstrong, K. F. (2006). DNA barcodes for insect pest identification: a test case with tussock moths (Lepidoptera: Lymantriidae). Canadian Journal of Forest Research, 36(2), 337-350.
  9. Barrett, R. D., & Hebert, P. D. (2005). Identifying spiders through DNA barcodes. Canadian Journal of Zoology, 83(3), 481-491.
  11. Blacket, M. J., Semeraro, L., & Malipatil, M. B. (2012). Barcoding Queensland fruit flies (Bactrocera tryoni): impediments and improvements. Molecular Ecology Resources, 12(3), 428-436.
  13. Ciceoi, R., Dobrin, I., Mardare, E. Ş., Dicianu, E. D., & Stănică, F. (2017). Emerging pests of Ziziphus jujuba crop in Romania. Scientific Papers. Series B, Horticulture, 61, 143-153.
  14. Clarke, A., Armstrong, K., Carmichael, A., Milne, J., Raghu, S., Roderick, G., & Yeates, D. (2005). Invasive phytophagous pests arising through a recent tropical evolutionary radiation: the Bactrocera dorsalis complex of fruit flies. Annual Review of Entomology, 50, 293-319.
  16. Dasmahapatra, K. K., Elias, M., Hill, R. I., Hoffman, J. I., & Mallet, J. (2010). Mitochondrial DNA barcoding detects some species that are real, and some that are not. Molecular Ecology Resources, 10(2), 264-273.
  18. De la Rúa, P., Jaffé, R., Dall'Olio, R., Muñoz, I., & Serrano, J. (2009). Biodiversity, conservation and current threats to European honeybees. Apidologie, 40(3), 263-284.
  20. El Harym, Y., & Belqat, B. (2017). First checklist of the fruit flies of Morocco, including new records (Diptera, Tephritidae). ZooKeys, 702, 137.
  22. Farrar, N., Asadi, G., & Golestaneh, S. (2004). Damage and host ranges of ber fruitfly Carpomya vesuviana Costa (Diptera: Tephritidae) and its rate of parasitism. Journal of Agriculture Sciences, 1(5), 120-130. (In Persian with English abstract).
  23. Folmer, O. F., Black, M. B., Hoeh, W. R., Lutz, R. V., & Vrijenhoek, R. C. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 35, 294-299.
  24. Ghanim, N., Abdel-Baky, N., Al-Doghairi, M., & Fouly, A. (2014). Evaluation of some ammonium compounds as olfactory stimulants for Zizyphus fruit fly Carpomya incompleta (Diptera: Tephritidae) in christ's thorn orchards at Qassim, Saudi Arabia. Journal of Plant Protection and Pathology, 5(4), 367-377.
  26. Hebert, P. D., Penton, E. H., Burns, J. M., Janzen, D. H., & Hallwachs, W. (2004). Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences, 101(41), 14812-14817.
  28. Ide, T., Kanzaki, N., Ohmura, W., & Okabe, K. (2016). Molecular identification of an invasive wood-boring insect Lyctus brunneus (Coleoptera: Bostrichidae: Lyctinae) using frass by loop-mediated isothermal amplification and nested PCR assays. Journal of Economic Entomology, 109(3), 1410-1414.
  30. Ismael, B. F., Abd, A. K. M., & Jabbar, F. J. (2022). Study the effect of antioxidants on the traits of the fruits of two cultivars of Jujube (Ziziphus mauritiana Lamk.) Al-Tufahi and Alarmouti Cultivars. Basrah Journal of Agricultural Sciences, 35(1), 1-20.
  32. Jebbar, A. (1996). Biological, ecological and behavior studies of tow species of ber fruit fly (Tephritidae: Diptera) in Basrah. Ph. D. thesis. Basrah University, 158pp. (In Arabic with English abstract).
  33. Kapoor, V. (2002). Fruit fly pests and their present status in India. Proceedings of the 6th International Symposium on Fruit Flies of Economic Importance, Stellenbosch, South Africa. Isteg Scientific Publications, 23-33p.
  34. Korneyev, V., Mishustin, R., & Korneyev, S. (2017). The Carpomyini fruit flies (Diptera: Tephritidae) of Europe, Caucasus, and Middle East: new records of pests, with improved keys. Vestnik zoologii, 51(6), 453.
  36. Korneyev, V. A., & Dirlbek, J. (2000). The fruit flies (Diptera: Tephritidae) of Syria, Jordan and Iraq. Studia Dipterologica, 7(2), 463-482.
  38. Mayhew, P. J. (2018). Explaining global insect species richness: lessons from a decade of macroevolutionary entomology. Entomologia experimentalis et applicata, 166(4), 225-250.
  40. Menabit, S., Iancu, L., Pavel, A. B., Popa, A., Lupascu, N., & Purcarea, C. (2022). Molecular identification and distribution of insect larvae in the Lower Danube River. Oceanological and Hydrobiological Studies, 51(1), 74-89.
  42. Mumford, J. (2005). Integrated management of fruit flies (Diptera: Tephritidae) in India. DFID Crop Protection Programme. 190pp.
  43. Nagoshi, R. N., Brambila, J., & Meagher, R. L. (2011). Use of DNA barcodes to identify invasive armyworm Spodoptera species in Florida. Journal of Insect Science, 11(1), 154.
  45. Navajas, M., & Fenton, B. (2000). The application of molecular markers in the study of diversity in acarology: A review. Experimental & Applied Acarology, 24(10), 751-774.
  47. Norrbom, A. (1997). The genus Carpomya Costa (Diptera: Tephritidae): new synonymy, description of first American species, and phylogenetic analysis. Proceedings of the Entomological Society of Washington, 99, 338-347.
  49. Parchami-Araghi, M., Gilasian, E., & Basavand, F. (2015). First record of jujube fruit fly Carpomya incompleta (Becker) (Diptera: Tephritidae) from Iran. Studia dipterologica, 22, 163-164.
  50. Tahir, H. M., Noor, A., Mehmood, S., Sherawat, S. M., & Qazi, M. A. (2018). Evaluating the accuracy of morphological identification of insect pests of rice crops using DNA barcoding. Mitochondrial DNA Part B, 3(2), 1220-1224.
  52. Van der Bank, H. F., Greenfield, R., Daru, B. H., & Yessoufou, K. (2012). DNA barcoding reveals micro-evolutionary changes and river system-level phylogeographic resolution of African silver catfish, Schilbe intermedius (Actinopterygii: Siluriformes: Schilbeidae) from seven populations across different African river systems. Acta Ichthyologica et Piscatoria, 42(4), 307-320.
  54. Zakari-Moussa, O., Ratnadass, A., Vayssières, J.-F., Nikiema, A., Fatondji, D., Salha, H., Aboubacar, K., Ryckewaer, P., Pasternak, D. (2012). GF-120 effects on fruit fly species (Diptera: Tephritidae) in Sahelian agroforestry-based horticultural cropping systems. Fruits, 67(5), 333-339.

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