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The goal of this study was to investigate the effect of varying water quality with and without vitamin C on the growth performance and some haematological parameters of broiler chickens. A total of 252 one day old, unsexed chicks of broiler chickens (ROSS 308), average body weight 42 g were used in this study. For 35 days, the chicks were divided into 7 treatments (each treatment thirty six chicks), with 3 replicate per each (twelve chicks per replicate). The first group drank reverse osmosis (R.O) without any addition containing 28.16 total dissolved solids mg.l-1 (TDS), whereas the second, third and fourth groups (Safwan, Qurna and Fao tap water) drank water containing 1849.6, 1452.8 and 1356.8 TDS mg.l-1 respectively. The fifth, sixth and seventh group drank water containing 1849.6, 1452.8 and 1356.8 TDS mg.l-1 with 100 mg.l-1 vitamin C respectively. The highest body weight, weight gain, feed intake and the better feed conversion ratio were observed at the seventh group. The highest cumulative water intake was observed at the second, third and fourth groups. Higher red blood cells (RBC), haemoglobin concentration (Hb), packed cells volume (PCV) and lymphocytes ratio were recorded at the seventh group. Higher heterophils and heterophils/lymphocytes ratio were observed at the second group. Mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were not affected by different water quality. It can be concluded that the increase of TDS in the drinking water to 1849.6 mg.l-1 led to a decline in the growth performance and haematological parameters, while the addition of vitamin C (100 mg.l-1) improved the growth parameters and physiological status of broilers.


Ascorbic acid Blood Broiler chickens Growth performance TDS

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Hussain, S. J. ., & Al-Salhie, K. C. K. . (2022). Effect of Water Quality and Vitamin C on the Growth Performance and Haematological Parameters of Broiler Chickens. Basrah Journal of Agricultural Sciences, 35(2), 248–258.


  1. Adenkola A. Y., & Angani, M. T. (2017). Ascorbic acid supplementation effect on hematology and oxidative stress parameters of broiler chicken during the hot-dry season in southern Guinea Savannah. Journal of Poultry Research, 14(1), 28-33.
  3. Ahmadu, S., Mohammed, A. A., Buhari, H., & Auwal A. (2016). An overview of vitamin C as an antistress in poultry. Malaysian Journal of Veterinary Research, 7(2), 9-22.
  4. Alahgholi, M., Tabeidian, S. A., Toghyani, M., & Ale Saheb Fosoul, S. S. (2014). Effect of betaine as an osmolyte on broiler chickens exposed to different levels of water salinity. Archives Animal Breeding, 57(1), 1-12.
  6. Al-Mosawy, A. M. S., & Al-Salhie, K. C. K. (2021). The Effect of alcoholic extract of rhizomes of greater Galangal (Alpinia galanga L.) on the serum antioxidant enzymes for japanese quail during oxidative stress induced by hydrogen peroxide. Basrah Journal of Agricultural Sciences, 34(1), 171-179.
  7. Atekwana, E. A., Atekwana, E. A., Rowe, R. S., Dale Werkema, J. R., & Franklyn, D. L. (2004). The relationship of total dissolved solids measurements to bulk electrical conductivity in an aquifer contaminated with hydrocarbon. Journal of Applied Geophysics, 56, 281-294.
  8. Bains, B. S. (1996). The role of Vitamin C in stress management. World Poultry, 12(4), 38-41.
  9. Blumenthal, U. J., Cifuentes, E., Bennett, S., Quigley, M., & Ruiz-Palacios, G. (2001). The risk of enteric infections associated with wastewater reuse: the effect of season and degree of storage of wastewater. Transactions of the Royal Society of Tropical Medicine and Hygiene, 95(2), 131-137.
  11. Campbell, T. W. (1995). Avian hematology and cytology. Iowa State University Press, 104pp.
  13. Chen. J., & Balnave, D. (2001). The influence of drinking water containing sodium chloride on performance and eggshell quality of a modern, colored layer strain. Poultry Science, 80(1), 91-94.
  15. Cinar, M., Yildirim, E., Yigit, A. A., Yalcinkaya, I., Duru, O., Kisa, U., & Atmaca, N. (2014). Effects of dietary supplementation with vitamin C and vitamin E and their combination on growth performance, some biochemical parameters, and oxidative stress induced by copper toxicity in broilers. Biological Trace Element Research, 158, 186-196.
  17. Crompton, T. R. (1997). Toxicants in the aqueous ecosystem, Chichester: John Wiley and Sons, Cambridge University Press, 382p.
  19. Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics, 11, 1-42.
  21. Erener, G., Ocak, N., & Ozdas, A. (2002). Effect of sodium chloride supplementation provided through drinking water and/or feed on performance of Japanese quails (Coturnix coturnix Japonica). Turkish Journal of Veterinary and Animal Sciences, 26, 1081-1085.
  23. Fails, A. D., & Magee, C. (2018). Anatomy and physiology of farm animals, Wiley Blackwell. 8th edition, 592pp.
  24. Geerling, J. C., & Loewy, A. D. (2008). Central regulation of sodium appetite. Experimental Physiology, 93, 177-209.
  26. Guyton, A. C., & Hall, J. E. (2021). Textbook of Medical Physiology, 14th Edition. 1152pp.
  28. Hashem, M. A., Abd El Hamied, S. S., Ahmed, E. M. A., Amer, S. A., & Hassan, A. M. (2021). Alleviating effects of vitamins C and E supplementation on oxidative stress, hematobiochemical, and histopathological alterations caused by copper toxicity in broiler chickens. Animals, 11(6), 1739.
  30. Honarbakhsh, S., Zaghari, M., & Shivazad, M. (2007). Interactive effects of dietary betaine and saline water on carcass traits of broiler chicks. Journal of Biological Sciences, 7(7), 1208-1214.
  32. Kellems, R. O., & Church, D. C. (2002). Livestock Feeds and Feeding. 5th Edn. Prentice Hall, Upper Saddle River, New Jersey.
  33. feeding/oclc/248198121?referer=di&ht=edition
  34. Kirkpatrick, K., & Fleming, E. (2008). ROSS TECH 08/47: Water Quality, Pp: 10.
  36. Khalilipour, G., Maheri Sis, N., & Shaddel-Teli, A. (2019). Effects of saline drinking water on carcass characteristics and litter moisture content of Japanese Quails (Coturnix coturnix japonica). Egyptian Journal of Veterinary Sciences, 50(2), 151-157.
  37. Lohakare, J. D., Ryu, M. H., Hahn T. W., Lee, J. K., & Chae, B. J. (2005). Effects of supplemental ascorbic acid on the performance and immunity of commercial broilers. Journal of Applied Poultry Research. 14(1), 10-19.
  39. Mahmud, A., Hayat, Z., Zafarullah Khan, M., Khalique, A., & Younus, M. (2010). Comparison of source and levels of sodium in broilers under low temperature conditions. Pakistan Journal of Zoology, 42, 383-388.
  40. Mahjar, N. T., & Al-Salhie, K. C. K. (2022). The effects of In ovo injection of Garlic (Allium sativum L.) extract on hatchability, liver enzymes and antioxidant status of broiler chickens. Basrah Journal of Agricultural Sciences, 35(1), 61-70.
  42. N.R.C. (1994). National Research Council. Nutrient Requirements of Poultry. 9th edn. Washington, D. C., 176pp.
  44. Obead, F. I., & Jerry, A. N. (2019). Effect of irrigation water quality and spraying with tocopherol on Na, K, K+/Na+ and Cl- and chemical components of Okra (Abelmoschus esculentus L. Moench). Basrah Journal of Agricultural Sciences, 32, 291-301.
  46. Rajput, A. B., Kolte, B. R., Shisodiya J. M., Chandankhede, J. M., & Chahande J. M. (2009). Effect of Viatmin A, Vitamin C, Vitamin E and levamisole on performance of broilers. Veterinary World, 2(6), 225-227.
  48. Saiz del Barrio, A., Mansilla, W. D., Navarro-Villa, A., Mica, J. H., Smeets, J. H. den Hartog, L. A., & García-Ruiz, A. I. (2020). Effect of mineral and vitamin C mix on growth performance and blood Corticosterone concentrations in heat-stressed broilers. Journal of Applied Poultry Research, 29, 23-33.
  50. Sahin, K. & Kucuk, O. (2001). Effect of vitamic C and vitamic E on performance digestion of nutrient and carcass characteristic of Japanese quails reared under chronic heat stress (34°C). Journal of Animal Physiology and Animal Nutrition, 85(11-12), 335-341.
  52. Scanes, C. G. & Dridi, S. (2021). Sturkie's Avian Physiology. Seventh Edition, ‎ Academic Press, 4509pp.
  54. SPSS (2016). Statistical Packages of Social Sciences. IBM Corp. Released 2016. IBM SPSS Statistics for Windows, Version 24.0. Armonk, NY: IBM Corp.
  55. https:// www. ibm. com/ analytics/spss-statistics-software
  56. Vosooghi-Postindoz, V., Tahmasbi, A., Naserian, A. A., Valizade, R., & Ebrahimi, H. (2018). Effect of water deprivation and drinking saline water on performance, blood metabolites, nutrient digestibility, and rumen parameters in Baluchi lambs. Iranian Journal of Applied Animal Science, 8(3), 445-456.
  58. Watkins, S. E., Fritts, C. A., Yan, F., Wilson, M. L,. & Waldroup, P. W. (2005). The interaction of sodium chloride levels in poultry drinking water and the diet of broiler chickens. Journal of Applied Poultry Research, 14(1), 55-59.
  60. Watson, K., Wiedemann, S., & McGahan, E. (2020). Industry best practice manual for water quality management and sterilisation on-farm. AgriFutures Australia Publication No. 20-088. AgriFutures Australia Project No. PRJ-011587, 110pp.
  62. Wei, J., Lei, G., Fu, L., Zeng, C., Yang, T., & Peng, S. (2016). Association between dietary vitamin C intake and non-alcoholic fatty liver disease: A cross-sectional study among middle-aged and older adults. Plos one, 11, e0147985.
  64. WHO, World Health Organization (2008). Guidelines for Drinking-water Quality. Third edition incorporating the first and second addenda Volume 1, Geneva.