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High seed quality and growth performance is farmer’s and agricultural enterprise’s demand. However, increasing population increases food demand and due to global warming and limited natural resources, more attention has been paid to implementing different effective strategies. Halopriming and hormopriming have shown improvement in different growth and developmental stages in plants and under adverse environmental conditions. Therefore, this study was aimed to study the interaction impact of halopriming and hormopriming on growth and seed production of faba bean (Vicia faba L.). The interaction effect of seed priming was examined during germination and field growth performance of faba bean. Different salt substances NaCl (0, 25 and 50 mM) and CaSO4 (0, 2.5 and 5 mM) were used as a halopriming. Salicylic acid (SA) (0, 0.5 and 1mM) was used as a hormopriming agent. Split-plot randomize complete block design was followed with three replicates. The results show a significant increase in seed germination when the seeds were haloprimed with CaSO4 (2.5 and 5 mM) and compared with the control. NaCl (25 and 50 mM) increased the number of fruit set, brunches, pods and yield per plant significantly in comparison to the control. SA at 0.5 mM increased plant height significantly when compared with the control. These results may indicate that different varieties and species respond differently to seed priming. In addition, the interaction between the halopriming and hormopriming compounds may function as a buffer to modify the osmotic presser during imbibition, and may the seed coat of fabe bean, Aquadlge, variety prevent the transport of the compounds to the embryo. Therefore, it has been suggested and recommended to implement a combination between halopriming and hormopriming using different species and faba bean variety and study its effect at the molecular level during seed germination.


Halopriming Hormopriming Seed quality Vicia faba L.

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How to Cite
Kka, N. M. E. ., Sharif, K. N. ., Mohammed, S. F. ., & AbdulHamid, K. A. . (2022). Interactions between Halopriming and Hormopriming in Regulating the Vegetative Growth and Seed Quality of Vicia faba L. Basrah Journal of Agricultural Sciences, 35(2), 1–23.


  1. Afzal, I., Rauf, S., Basra, S. M. A., & Murtaza, G. (2008). Halopriming improves vigor, metabolism of reserves and ionic contents in wheat seedlings under salt stress. Plant Soil Environment, 54(9), 382-388.
  3. Ahmad, I., Saleem, A. M., Mustafa, G., Ziaf, K., Afzal, I., & Qasim, M. (2017). Seed halopriming enhances germination performance and seedling vigor of gerbera jamesonii and zinnia elegans. Sarhad Journal of Agriculture, 33(2), 199-205.
  5. Anaya, F., Fghire, R., Wahbi, S., & Loutfi, K. (2018). Influence of salicylic acid on seed germination of Vicia faba L. under salt stress. Journal of the Saudi Society of Agricultural Sciences, 17(1), 1-8.
  7. Anonymous (2021). Monthly weather forecast and climate Erbil, Iraq. Weather Atlas.
  9. Azooz, M. (2009). Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes differing in salt tolerance. International Journal Of Agriculture & Biology, 11(4), 343-350.
  11. Bradford, K. J. (1990). A water relations analysis of seed germination rates. Plant Physiology, 94(2), 840-849.
  13. Damalas, C. A., Koutroubas, S. D., & Fotiadis, S. (2019). Hydro-priming effects on seed germination and field performance of faba bean in spring sowing. Agriculture, 9(9), 201.
  15. del Pilar Cordovilla, M., Ligero, F., & Lluch, C. (1995). Influence of host genotypes on growth, symbiotic performance and nitrogen assimilation in faba bean (Vicia faba L.) under salt stress. Plant and Soil, 172(2), 289-297.
  17. Farooq, M., Usman, M., Nadeem, F., Rehman, H. U., Wahid, A., Basra, S. M. A., & Siddique, K. H. M. (2019). Seed priming in field crops: potential benefits, adoption and challenges. Crop and Pasture Science, 70(9), 731-771.
  19. Gadallah, M. A. A. (1999). Effects of proline and glycinebetaine on vicia faba responses to salt stress. Biologia Plantarum, 42(2), 249-257.
  21. Garcia, D., Arif, S., Zhao, Y., Zhao, S., Ming, L. C., & Huang, D. (2021). Seed priming technology as a key strategy to increase crop plant production under adverse environmental conditions. Preprints, 2021, 2021090364.
  23. Hussain, S., Khan, F., Hussain, H. A., & Nie, L. (2016). Physiological and biochemical mechanisms of seed priming-induced chilling tolerance in rice cultivars. Frontiers in Plant Science, 7, 116, 1-14
  24. Jawad, H. (2020). Spatial relationships of soil salinity in Iraq and its impact agricultural production. Al-Mostansiriyah Journal for Arab and International Studies, 7(72), 243-259.
  25. (In Arabic).
  26. Jisha, K. C., & Puthur, J. T. (2014). Halopriming of seeds imparts tolerance to NaCl and PEG induced stress in Vigna radiata (L.) Wilczek varieties. Physiology and Molecular Biology of Plants, 20(30), 303-312.
  28. Karim, M. N., Sani, M. N. H., Uddain, J., Azad, M. O. K., Kabir, M. S., Rahman, M. S., Choi, K. Y., & Naznin, M. T. (2020). Stimulatory effect of seed priming as pretreatment factors on germination and yield performance of yard long bean (Vigna unguiculata). Horticulturae, 6(4), 104.
  30. Kumari, N., Rai, P. K., Bara, B. M., Singh, I., & Rai, K. (2017). Effect of halo priming and hormonal priming on seed germination and seedling vigour in maize (Zea mays L.) seeds. Journal of Pharmacognosy Phytochemistry, 6(4), 27-30.
  32. Majeed, A., & Muhammad, Z. (2019). Salinity: A major agricultural problem-causes, impacts on crop productivity and management strategies. Pp, 83-99. In: Hasanuzzaman M., Hakeem K., Nahar, K., & Alharby, H. (Eds). Plant Abiotic Stress Tolerance. Springer, Cham., 506pp.
  34. Manonmani, V., Begum, M., & Jayanthi, M. (2014). Halo priming of seeds. Research Journal of Seed Science, 7(1), 1-13.
  36. Mazibuko, T. G., & Modi, A. T. (2005). Comparison of osmopriming and seed coating with calcium salts for green bean performance under field conditions. I. Cotyledonal cracking. South African Journal of Plant and Soil, 22(1), 9-15.
  37. Mustafa, G., Masood, S., Ahmed, N., Saboor, A., Ahmad, S., Hussain, S., Bilal, M., & Ali, M. A. (2019). Seed Priming for Disease Resistance in Plants. Pp, 333-362. In: Hasanuzzaman, M., Fotopoulos, V. (eds) Priming and Pretreatment of Seeds and Seedlings. Springer, Singapore, 606pp.
  39. Nabi, F., Chaker-Haddadj, A., Chebaani, M., Ghalem, A., Mebdoua, S., & Ounane, S. (2020). Influence of seed priming on early stages growth of cowpea [Vigna unguiculata (L.) Walp.] grown under salt stress conditions. Legume Research, 2020(43), 665-671.
  41. Nadeem, M., Li, J., Yahya, M., Wang, M., Ali, A., Cheng, A., Wang, X., & Ma, C. (2019). Grain legumes and fear of salt stress: focus on mechanisms and management strategies. International Journal of Molecular Sciences, 20(4), 799.
  43. Parihar, P., Singh, S., Singh, R., Singh, V. P., & Prasad, S. M. (2015). Effect of salinity stress on plants and its tolerance strategies: A review. Environmental Science and Pollution Research, 22(6), 4056-4075.
  45. Qados, A. M. A. (2011). Effect of salt stress on plant growth and metabolism of bean plant Vicia faba (L.). Journal of the Saudi Society of Agricultural Sciences, 10(1), 7-15.
  47. Qureshi, A., & Al-Falahi, A. (2015). Extent, characterization and causes of soil salinity in central and southern Iraq and possible reclamation strategies. International Journal of Engineering Research and Applications, 5(1), 84-94.
  49. Rabie, G., & Almadini, A. (2005). Role of bioinoculants in development of salt-tolerance of Vicia faba plants under salinity stress. African Journal of Biotechnology, 4(2), 210-222.