Article Sidebar

Download
PDF
Statistic
Read Counter : 250 Download : 84

Downloads

Download data is not yet available.

Main Article Content

Abstract

This study was conducted on shoot tip explants of date palm cv. Barhee to investigate effect of bacterial cell wall components on initial callus growth. Three concentrations of bacterial media ( 0.5 and 1.0 and 2.5) v/v were used for growing callus by tissue culture technique . MS media was served as a control treatment.Results showed that all bacterial media treatments increased callus fresh weight and nitrogen concentration of media significantly as compared to that of MS control media. Bacterial media treatment of 2.5 (v/v) gave the highest values of callus fresh weight and nitrogen concentration (518 mg and 3.3089%) respectively, whereas MS control media had the lowest values (193 mg and 0.01%) respectively. There was a significant increase in callus fresh weight at 60 days incubation over that of 30 days incubation. The combination of bacterial media treatment at 2.5 (v/v) and incubation period of 60 days recorded the highest significant increase in callus fresh weight (631 mg), whereas the combination of MS control media treatment and incubation period of 30 days had the lowest value (166 mg) in this respect.

Keywords

Phoenix dactylifera L. Barhee. In vitro bacterial cell wall initial pro callus nitrogen

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite
Attaha, A. H., Abdul-Sada, H. K., & Al-Mussawii, M. A. (2017). Effect of Bacterial Cell Wall Components on Callus Growth of Phoenix dactylifera L. cv. Barhee Propagated Through In vitro. Basrah Journal of Agricultural Sciences, 30(1), 1–6. https://doi.org/10.37077/25200860.2017.5
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Abd El-Zaher, M.H. (2008). Studies on micro propagation of Jackfruit: 2-A comparative histological studies on in vitro and ex vitro plants of Jackfruit. World Journal of Agricultural Sciences, 4(2): 255-262.
  2. Abdel-Rahim, E. A.; Abdel-Fatah, O.M.; Kobasse, M. I.; El-Shemy, H.A. and Abd El-Samei, M.B. (1998). Growth of date palm callus as affected by growth regulators, sugars as carbon source and amino acids as organic nitrogen source. Arab Journal of Biotechnology, 1(1): 99-106.
  3. Abou El-Nil, M. and Al-Ghamdi, A.S. (1986). Stimulation of growth and tissue culture of date palm axillary buds by injection of offshoot with a cytokinin. Proceedings of the 2nd Symposium on Date Palm, King Faisal Univ., Al-Hassa Saudi Arabia,1: 43-44.
  4. Al-Khayri, J.M. (2001). Optimization of biotin and thiamine requirements for somatic embryogenesis of date palm (Phoenix dactylifera L.). In vitro Cell. Dev. Biol. Plant, 37: 453-456.
  5. Behrend, J. and Mateles R.I. (1975). Nitrogen metabolism in plant cell suspension cultures.1. Effect of amino acids on growth. Plant Physiol., 56: 584-589.
  6. Benson, E.E. (2000). In vitro plant recalcitrance, an introduction. In Vitro Cell. Dev. Biol. Plant, 36: 141-148.
  7. Bhojwani, S.S., and Razdan. M.K. (1983). Plant tissue culture: Theory and practice. Elsevier Pub. House, Amsterdam. 502pp.
  8. Cresser, M.S., and Parsons, J.W. (1979). Sulphuric-perchloric acid and fruit quality of bartamoda date palm propagated by tissue culture technique under Aswan conditions. Journal of Applied Sciences Research, 6(2): 184-190.
  9. Cummins, C.S. (1970). Cell wall composition in the classification of gram positive anaerobes. International Journal of Systematic Bacteriology, 20(4): 413-419.
  10. El-Din, Z.; Amal, F.M.; Abd El-Rasoul, M.; Ibrahim, I.S.; Aly, A.S. and Sharaf Eldeen, H.A.M. (2007). Micropropagation of some date palm cultivars: Changes of some chemical constituents related to embryogenesis. ISHS Acta Horticulturae, 3rd. Int. Date Palm Conf. (Abs.).
  11. Hediger, S.; Bardet, M. and Giffard, M. (2013). Structure and dynamics of the bacterial cell well: A solid-state NMR study. Institute for Nanoscience and Cryogenics. 568pp.
  12. Joseph, C.P. (1976). Nitrogen metabolism in soybean tissue culture: Assimilation of urea. Plant Physiol., 58: 350-351.
  13. Khlifi, S. and Tremblay, F.M. (1995). Maturation of black spruce somatic embryos. Part I. Effect of glutamine on the number and germ inability of somatic embryos. Plant Cell, Tiss. Org. Cult., 41: 23-32.
  14. Mohamed, M.S. (1996). Biochemical studies on fenugreek by using tissue culture techniques. M. Sc. Thesis, Fac. Agric., Univ. Cairo.
  15. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15: 473-497.
  16. Olrichs, N.K. (2010). Bugging the cell wall of bacteria: Novel insights into the biosynthesis of peptidoglycan and its inhibition. Scheikunde Roefschriften, Lectures, No. 54. Utrecht University. (Abs.).
  17. Osman, S.M. (2010). Effect of potassium fertilization on yield, leaf mineral content and fruit quality of bartamoda date palm propagated by tissue culture technique under Aswan conditions. Journal of Applied Sciences Research, 6(2): 184-190.
  18. Page, A.L.; Miller, P.H. and Keeney, D.R. (1982). Methods of Soil Analysis. Part (2). Chemical and Microbiological Propertie. Agron. Series No. 9. Amer. Soc. Agron. Inc. Pub. Madison. Wisconsin. Pp: 1324-1341.
  19. Singh, N.N.; Kokate, C.K., and Tipnis, H.P. (1981). A note on development of callus cultures of Trigonella foenumgraecum for diosgenin biproduction. Indian Drugs, 19(1): 25.
  20. Steinhart C.E.; Standifer, L.C. and Skoog,F. (1961). Nutrient requirements for in- vitro growth of callus tissue. American Journal of Botany, 48(6-Part, 1): 465.