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Abstract
The aim of the study was to detect polymorphism in the POU1F1 gene in Iraqi Awassi sheep breed, as well as to establish if haplotype of POU1F1 gene could be associated with productive traits. This study was carried out at Al-Kafeel station, Karbala city during the period of 01/10/2017 until 01/08/2018. The study included 46 Awassi ewes with their 52 lambs. The laboratory analyses were conducted at the Laboratory of Molecular Genetics, College of Agriculture, University of Basrah. Results showed the successfulness of the PCR amplification process for all six examined fragments of the POU1F1 gene. Gel electrophoresis was conducted using agarose 2%, the product sizes were 637bp, 789bp, 999bp, 868bp, 1190 bp, and 469bp for the fragments P1, P2, P3, P4, P5, and P6 respectively. The analysis of the nitrogen bases sequences of the POU1F1 gene for the studied fragments showed a change in 12 different sites of the gene. These changes resulted in 7 haplotypes of H1-H7. The results showed significant influence (P<0.05) in haplotypes of the POU1F1 gene on birth weight . However, haplotypes showed no significant effect on lambs’ weights at weaning, six months and daily weight gains during all periods. Statistical analysis showed that different haplotypes of the POU1F1 gene did not influence on milk productive traits. The examined fragments of the POU1F1 Awassi gene have been submitted to Gene Bank under the accession numbers (LC469323 to LC469349).
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References
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References
Al-Khuzai, H.M. (2018). Detection of IGFBP-3, POUIFI and BMP15 genes and their association with the productive and reproductive performance for Awassi sheep. Ph. D. Thesis. Coll. Agric., Univ. Baghdad: 165pp.
Ansary, M.; Tahmoorespour, M.; Nassiry,R. & Javadmanesh, A. (2008). Study the PIT-1 gene polymorphism and association to average daily gain in Iranian Baluchi sheep. 10th World Congress on Anim. Prod. South Africa, 25: 23-28.
Aytekin, I. & Boztepe, S. (2013). Association of PIT-1 Gene polymorphism with milk yield and composition traits in brown Swiss cattle. J. Anim. Plant Sci., 23: 1281-1289.
Bastos, E.; Avila, S. Cravador, A.; Renaville, R.; Pinto, H.G. & Castrillo, J.L. (2006). Identification and characterization of four splicing variants of ovine POU1F1 gene. Gene., 382: 12-19.
Carrijo, S.M.; De Alencar, M.M.; Toral, F.L.B. & Reagitano, L.C., (2008) Association of PIT1 genotypes with growth traits in Canchim cattle. Sci. Agric. ., 65: 116-121.
Chauhan, A.; Tiwari, A.; Singh, S.P.; Sharma, D.; Kumar, S.; Goel, R.; Bhattachrya, A. & Singh, V. (2015). Association of PIT-1 gene polymorphism with milk production traits in Sahiwal cattle. Indian J. Anim. Sci., 85: 610-612.
Daga, C.; Paludo, M.; Luridiana, S.; Mura, M.C.; Bodano, S.; Pazzola, M.; Dettori, M.L.; Vacca, G.M. & Carcangiu, V. (2013). Identification of novel SNPs in the Sarda breed goats POU1F1 gene and their association with milk productive performance. Mol. Biol. Rep., 40: 2829-2835.
Gil-Puig, C.; Blanco, M.; García-Caballero, T.; Segura, C. & Fernández, R.P. (2002). Pit-1/GHF-1 and GH expression in the MCF-7 human breast adenocarcinoma cell line. J. Endocr., 173: 161-167.
Huang, W.; Maltecca, C. & Khatib, H.A. (2008). Proline-to-histidine mutation in POU1F1 is associated with production traits in dairy cattle Anim. Genet., 39: 554-557.
Mura, M.C.; Daga, C.; Paludo, S.L.; Pazzola, M.; Bodano, S.; Dettori, M.L.; Vacca, G.M. & Carcangiu, V. (2012). Analysis of polymorphism within POU1F1 gene in relation to milk production traits in dairy Sarda sheep breed. Mol. Biol. Rep., 39: 6975-6979.
Ozmen, O.; Kul, S. & Unal, E.O. (2014). Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits. Iranian J. Vet. Res., 15: 331-335.
Pan, C.Y.; Lan, Chen, H.; Yang, D.Y.; Hua, L.S.; Yang, X.B.; Lei, C.Z.; Gui, Y.K.; Zhang, B.; Zhang, C.L.; Kang, X.T. & Wang, I.Q. (2008). A DdeI PCR-RFLP detecting a novel missense mutation of the POU1F1 gene showed no e?ects on growth traits in cattle. Czech J. Anim. Sci., 12: 523-527.
Sadeghi, M.I.; Jalil-Sarghale, A. & Moradi-Shahrbabak, M. (2014).Associations of POU1F1 gene polymorphisms and protein structure
changes with growth traits and blood metabolites in two Iranian sheep breeds. J. Genet., 93: 831-835.
Seong, J.; Oh, J.D.; Cheong, I.C.; Lee, K.W.; Lee, H.K.; Suh, D.S.; Jeon, G.J.; Park, K.D. & Kong, H.S. (2011). Association between polymorphisms of Myf5 and POU1F1 genes with growth and carcass traits in Hanwoo (Korean cattle). Genes & Genomics, 33: 425-430.
Stasio, L.D.; Sartore, S. & Alberta, A. (2002). Lack of association of GH1 and POU1F1 gene variants with meat production traits in Piedmontese cattle. Anim. Genet., 33: 61-64.
SPSS, (2013). Statistical Package of Social Science .Ver. 22. Application Guide .Copy right by Spss Inc.
Sun, H.S.; Anderson, L.L.; Yu, T.P.; Kim, K.S.; Klindt, J. & Tuggle, C.K. (2002). Neonatal Meishan pigs show POU1F1 genotype e?ects on plasma GH and PRL concentration. Anim. Reprod. Sci., 69: 223-237.
Woollard, J.; Tuggle, C.K. & Leon, FA. (2000). Localization of POU1F1 to bovine, ovine, and caprine 1q21-22. J. Anim. Sci., 78: 242-243.
Zhang, C.; Liu, B.; Chen, H.; Lan, C.; Zhang, Z.; Zhang, R. & Shaaxi, P.R. (2013). Associations of a HinfI PCR-RFLP of POU1F1 Gene with Growth Traits in Qinchuan Cattle. Anim. Biot., 20: 71-74.
Zhu, H.; Zhang, Y.; Bai, Y.; Yang, H.; Yan, H.; Liu, J.; Shi, L.; Song, X.; Li, L.; Dong, S.; Pan, C.; Lan, X. & Qu, L. (2019). Relationship between SNPs of POU1F1 Gene and litter size and growth traits in shaanbei white cashmere goats. Anim., 9: 114. doi:10.3390/ani9030114.